Fast Sterility Assessment by Germinable-Endospore Biodosimetry
نویسندگان
چکیده
منابع مشابه
Fast sterility assessment by germinable-endospore biodosimetry.
The increased demand for sterile products has created the need for rapid technologies capable of validating the hygiene of industrial production processes. Bacillus endospores are in standard use as biological indicators for evaluating the effectiveness of sterilization processes. Currently, culture-based methods, requiring more than 2 days before results become available, are employed to verif...
متن کاملRadiation Dose Estimation by Automated Cytogenetic Biodosimetry.
The dose from ionizing radiation exposure can be interpolated from a calibration curve fit to the frequency of dicentric chromosomes (DCs) at multiple doses. As DC counts are manually determined, there is an acute need for accurate, fully automated biodosimetry calibration curve generation and analysis of exposed samples. Software, the Automated Dicentric Chromosome Identifier (ADCI), is presen...
متن کاملApplications of a rapid endospore viability assay for monitoring UV inactivation and characterizing arctic ice cores.
We have developed a rapid endospore viability assay (EVA) in which endospore germination serves as an indicator for viability and applied it to (i) monitor UV inactivation of endospores as a function of dose and (ii) determine the proportion of viable endospores in arctic ice cores (Greenland Ice Sheet Project 2 [GISP2] cores; 94 m). EVA is based on the detection of dipicolinic acid (DPA), whic...
متن کاملThe endospore of Bacillus polymyxa.
The endospore of Bacillus polymyxa was originally described by Meyer (1897) in a study of a bacillus which he designated Astasia asterospora. Meyer's drawings of the spore, showing ridges and the star shaped aspect, were reproduced by Knaysi (1951) over the name Bacillus asterosporus. It has not been generally recognized that this name is synonymous with B. polymyxa (Smith, et al., 1952). So th...
متن کاملAnti-Staphylococcal and Antifungal Substances Produced By Endospore-Forming Bacilli
Some bacteria and fungi are related to deterioration and also transmission of foodborne diseases, emphasizing the need to search new substances that may act in the treatment and prevention of the illnesses transmitted by food. Strains from genus Bacillus produce a variety of substances with inhibitory activity that range from antibiotics to bacteriocins. In this work, three strains, identified ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2008
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01437-08